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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SH3GLB2 Monoclonal antibody

SH3GLB2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

4B1A10

Cat no : 68325-1-Ig

Synonyms

Endophilin B2, KIAA1848, PP6569, PP9455, SH3GLB2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SH3GLB2 Monoclonal antibody (68325-1-Ig)

    WB analysis using 68325-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using SH3GLB2 Monoclonal antibody (68325-1-Ig)

    WB analysis using 68325-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunoprecipitation (IP) experiment of HeLa cells using SH3GLB2 Monoclonal antibody (68325-1-Ig)

    IP experiment of HeLa using 68325-1-Ig

    IP result of anti-SH3GLB2 (IP:68325-1-Ig, 4ug; Detection:68325-1-Ig 1:800) with HeLa cells lysate 1080 ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SH3GLB2 Monoclonal antibody (68325-1-Ig)

    IHC staining of mouse testis using 68325-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SH3GLB2 Monoclonal antibody (68325-1-Ig)

    IHC staining of mouse testis using 68325-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SH3GLB2 Monoclonal antibody (68325-1-Ig)

    IF Staining of HeLa using 68325-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SH3GLB2 antibody (68325-1-Ig, Clone: 4B1A10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inLNCaP cells, rat brain tissue, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, pig brain tissue, rabbit brain tissue, mouse brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68325-1-Ig targets SH3GLB2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Reactivity Human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SH3GLB2 fusion protein Ag8932 相同性解析による交差性が予測される生物種
Full Name SH3-domain GRB2-like endophilin B2
Calculated molecular weight 395 aa, 44 kDa
Observed molecular weight 47-50 kDa
GenBank accession numberBC014635
Gene symbol SH3GLB2
Gene ID (NCBI) 56904
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SH3GLB2 is an endophilin-related protein family with 395-amino acid protein and contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain. SH3GLB2 is expressed in many tissues, including skeletal muscle, adipocytes, brain, lung, colon, and mammary gland. SH3GLB2 was expressed in the cytoplasm of transfected HeLa cells and was excluded from nuclei(PMID: 11161816).

Protocols

Product Specific Protocols
WB protocol for SH3GLB2 antibody 68325-1-IgDownload protocol
IHC protocol for SH3GLB2 antibody 68325-1-IgDownload protocol
IF protocol for SH3GLB2 antibody 68325-1-IgDownload protocol
IP protocol for SH3GLB2 antibody 68325-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols