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  • KD/KO Validated

POSH Polyclonal antibody

POSH Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14649-1-AP

Synonyms

KIAA1494, Plenty of SH3s, POSH, Protein POSH, RING finger protein 142, RNF142, SH3 multiple domains protein 2, SH3MD2, SH3RF1


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Tested Applications

Positive WB detected inmouse brain tissue, DU 145 cells, HEK-293 cells, human brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14649-1-AP targets POSH in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, drosophila
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POSH fusion protein Ag6243 相同性解析による交差性が予測される生物種
Full Name SH3 domain containing ring finger 1
Calculated molecular weight 93 kDa
Observed molecular weight 93-100 kDa
GenBank accession numberBC053671
Gene symbol SH3RF1/POSH
Gene ID (NCBI) 57630
RRIDAB_2187290
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

POSH (plenty of SH3 domains) is a multidomain GTP-Rac1-interacting protein that acts as a scaffold for JNK activation, leading to apoptosis in neuronal and non-neuronal cells. It consists a RING-finger domain that possesses ubiquitin-ligase activity and four SH3 domains.

Protocols

Product Specific Protocols
WB protocol for POSH antibody 14649-1-APDownload protocol
IHC protocol for POSH antibody 14649-1-APDownload protocol
IP protocol for POSH antibody 14649-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
drosophilaIF

J Cell Biol

Rab8, POSH, and TAK1 regulate synaptic growth in a Drosophila model of frontotemporal dementia.

Authors - Ryan J H West
mouseWB

Int J Mol Sci

Dual Leucine Zipper Kinase Is Constitutively Active in the Adult Mouse Brain and Has Both Stress-Induced and Homeostatic Functions.

Authors - Sunil Goodwani
mouseIF

Hum Mol Genet

The Pro-Apoptotic JNK Scaffold POSH/SH3RF1 Mediates CHMP2BIntron5-Associated Toxicity in Animal Models of Frontotemporal Dementia.

Authors - Ryan J H West
human,mouseWB,IF,IP

Expert Opin Ther Targets

POSH participates in epileptogenesis by increasing the surface expression of the NMDA receptor: a promising therapeutic target for epilepsy.

Authors - Xinshi Wang
  • KD Validated
human,mouseWB,IP

Cell Rep

POSH regulates assembly of the NMDAR/PSD-95/Shank complex and synaptic function.

Authors - Minghui Yao
humanWB

Int J Biol Macromol

Snakehead vesiculovirus hijacks SH3RF1 for replication via mediating K63-linked ubiquitination at K264 of the phosphoprotein

Authors - Xiangmou Qin