SHANK1 Polyclonal antibody
SHANK1 Polyclonal Antibody for WB, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF-P, IP, ELISA
Conjugate
Unconjugated
Cat no : 55059-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, Neuro-2a cells |
| Positive IP detected in | HEK-293T cells |
| Positive IF-P detected in | mouse eye tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
55059-1-AP targets SHANK1 in WB, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | SH3 and multiple ankyrin repeat domains 1 |
| Calculated molecular weight | 225 kDa |
| Observed molecular weight | 159-225 kDa |
| GenBank accession number | NM_016148 |
| Gene symbol | SHANK1 |
| Gene ID (NCBI) | 50944 |
| RRID | AB_10863130 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SHANK1 belongs to the SHANK family. It seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. SHANK1 may play a role in the structural and functional organization of the dendritic spine and synaptic junction. The antibody is specific to SHANK1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SHANK1 antibody 55059-1-AP | Download protocol |
| IF protocol for SHANK1 antibody 55059-1-AP | Download protocol |
| IP protocol for SHANK1 antibody 55059-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Res METTL3-mediated N6-methyladenosine mRNA modification enhances long-term memory consolidation. | ||
Neural Regen Res Protective effects of Bushen Tiansui decoction on hippocampal synapses in a rat model of Alzheimer's disease. |
