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  • Featured Product
  • KD/KO Validated

SHARPIN Polyclonal antibody

SHARPIN Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14626-1-AP

Synonyms

SIPL1, Shank-interacting protein-like 1, Shank-associated RH domain-interacting protein, PSEC0216, hSIPL1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using SHARPIN Polyclonal antibody (14626-1-AP)

    WB analysis using 14626-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14626-1-AP (SHARPIN antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using SHARPIN Polyclonal antibody (14626-1-AP)

    WB analysis of Jurkat using 14626-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14626-1-AP (SHARPIN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using SHARPIN Polyclonal antibody (14626-1-AP)

    WB analysis of A549 using 14626-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 14626-1-AP (SHARPIN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SHARPIN Polyclonal antibody (14626-1-AP)

    WB analysis of mouse brain using 14626-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14626-1-AP (SHARPIN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using SHARPIN Polyclonal antibody (14626-1-AP)

    WB analysis of mouse skeletal muscle using 14626-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14626-1-AP (SHARPIN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Raji cells using SHARPIN Polyclonal antibody (14626-1-AP)

    IP experiment of Raji using 14626-1-AP

    IP result of anti-SHARPIN (IP:14626-1-AP, 3ug; Detection:14626-1-AP 1:1500) with Raji cells lysate 2520ug.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using SHARPIN Polyclonal antibody (14626-1-AP)

    IHC staining of human lymphoma using 14626-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14626-1-AP (SHARPIN Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using SHARPIN Polyclonal antibody (14626-1-AP)

    IHC staining of human lymphoma using 14626-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14626-1-AP (SHARPIN Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using SHARPIN Polyclonal antibody (14626-1-AP)

    IHC staining of human small intestine using 14626-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14626-1-AP (SHARPIN Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using SHARPIN Polyclonal antibody (14626-1-AP)

    IHC staining of human small intestine using 14626-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14626-1-AP (SHARPIN Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using SHARPIN Polyclonal antibody (14626-1-AP)

    IF Staining of Jurkat using 14626-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using SHARPIN antibody (14626-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inA549 cells, mouse brain tissue, mouse skeletal muscle tissue, Jurkat cells, Raji cells
Positive IP detected inRaji cells
Positive IHC detected inhuman lymphoma tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14626-1-AP targets SHARPIN in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHARPIN fusion protein Ag6201 相同性解析による交差性が予測される生物種
Full Name SHANK-associated RH domain interactor
Calculated molecular weight 40 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC025244
Gene symbol SHARPIN
Gene ID (NCBI) 81858
RRIDAB_2187734
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SHARPIN is as a third component of the linear ubiquitin chain assembly complex (LUBAC), recruited to the CD40 and TNF receptor signaling complexes together with its other constituents, HOIL1 and HOIP(PMID:21455173).SHARPIN is enriched in the postsynaptic density and forms a complex with SHANK in heterologous cells and brain(PMID:11178875).It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for SHARPIN antibody 14626-1-APDownload protocol
IHC protocol for SHARPIN antibody 14626-1-APDownload protocol
IF protocol for SHARPIN antibody 14626-1-APDownload protocol
IP protocol for SHARPIN antibody 14626-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

OTULIN limits cell death and inflammation by deubiquitinating LUBAC.

Authors - Klaus Heger
View Article
mouseWB,IP

Nature

LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis.

Authors - Nieves Peltzer
View Article
humanWB,IP

Cell

OTULIN Antagonizes LUBAC Signaling by Specifically Hydrolyzing Met1-Linked Polyubiquitin.

Authors - Keusekotten Kirstin K
View Article
humanWB

Cell

The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity.

Authors - Rune Busk Damgaard
View Article
mouseWB

Cancer Cell

A Dual Role of Caspase-8 in Triggering and Sensing Proliferation-Associated DNA Damage, a Key Determinant of Liver Cancer Development.

Authors - Yannick Boege
View Article
mouseWB

Nat Immunol

Pellino3 ubiquitinates RIP2 and mediates Nod2-induced signaling and protective effects in colitis.

Authors - Yang Shuo S
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Katharina (Verified Customer) (07-17-2019)

The antibody worked as expected, detecting a nice band at the right size. Recommended

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: A549 cells /immunoprecipitates