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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SHARPIN Monoclonal antibody (67177-1-Ig)

    WB analysis using 67177-1-Ig (same clone as 67177-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67177-1-Ig (SHARPIN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67177-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SHARPIN Monoclonal antibody (67177-1-Ig)

    WB analysis using 67177-1-Ig (same clone as 67177-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67177-1-Ig (SHARPIN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67177-1-PBS in a different storage buffer formulation.

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SHARPIN Monoclonal antibody, PBS Only

SHARPIN Monoclonal Antibody for WB, Indirect ELISA
Cat No. 67177-1-PBS
Clone No.3A9E2

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, Indirect ELISA

Synonyms

DKFZp434N1923, hSIPL1, SHARPIN, SIPL1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67177-1-PBS targets SHARPIN in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SHARPIN fusion protein Ag6367 相同性解析による交差性が予測される生物種
Full Name SHANK-associated RH domain interactor
Calculated molecular weight 40 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC025244
Gene Symbol SHARPIN
Gene ID (NCBI) 81858
RRIDAB_2882473
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H0F6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

SHARPIN is as a third component of the linear ubiquitin chain assembly complex (LUBAC), recruited to the CD40 and TNF receptor signaling complexes together with its other constituents, HOIL1 and HOIP(PMID:21455173).SHARPIN is enriched in the postsynaptic density and forms a complex with SHANK in heterologous cells and brain(PMID:11178875).It has 2 isoforms produced by alternative splicing.