SHBG Polyclonal antibody
SHBG Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 18200-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human plasma |
| Positive IHC detected in | mouse testis tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
18200-1-AP targets SHBG in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SHBG fusion protein Ag12683 相同性解析による交差性が予測される生物種 |
| Full Name | sex hormone-binding globulin |
| Calculated molecular weight | 402 aa, 44 kDa |
| Observed molecular weight | 42-44 kDa |
| GenBank accession number | BC101785 |
| Gene symbol | SHBG |
| Gene ID (NCBI) | 6462 |
| RRID | AB_2878517 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SHBG (sex hormone-binding globulin), also known as androgen-binding protein (ABP), is the specific transport protein for sex steroid hormones in humans. SHBG is synthesized mainly by the liver and circulates in the blood, in form of homodimer with a single steroid-binding site per dimer. In addition to the plasma SHBG, a testis isoform expressed by Sortoli cells also exists due to the gene alternative splicing. Various bands (44-56 kDa) recognized by this antibody may represent variably glycosylated or different isoforms of SHBG.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SHBG antibody 18200-1-AP | Download protocol |
| IHC protocol for SHBG antibody 18200-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Endocrinol FSH inhibits autophagy and lysosomal biogenesis to regulate protein degradation in cultured goat Sertoli cells. | ||
Biol Reprod Changes in histology, protein expression, and autophagy in dairy goat testes during non-breeding season. | ||
Theriogenology FSH-inhibited autophagy protects against oxidative stress in goat Sertoli cells through p62-Nrf2 pathway |
