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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SHBG Polyclonal antibody

SHBG Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31025-1-AP

Synonyms

ABP, SBP, sex hormone binding globulin, Sex steroid binding protein, SHBG, TEBG

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using SHBG Polyclonal antibody (31025-1-AP)

    WB analysis of human placenta using 31025-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 31025-1-AP (SHBG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inhuman placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31025-1-AP targets SHBG in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name sex hormone-binding globulin
Calculated molecular weight 402 aa, 44 kDa
Observed molecular weight40-50 kDa
GenBank accession numberBC101785
Gene symbol SHBG
Gene ID (NCBI) 6462
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SHBG (sex hormone-binding globulin), also known as androgen-binding protein (ABP), is the specific transport protein for sex steroid hormones in humans. SHBG is synthesized mainly by the liver and circulates in the blood, in form of homodimer with a single steroid-binding site per dimer. In addition to the plasma SHBG, a testis isoform expressed by Sortoli cells also exists due to the gene alternative splicing. Various bands (44-56 kDa) recognized by this antibody may represent variably glycosylated or different isoforms of SHBG.

Protocols

Product Specific Protocols
WB protocol for SHBG antibody 31025-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols