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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SHC1 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.156-20 ng/mL

Sandwich ELISA: 125-4000 pg/mL

Cat No : MP01619-2

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP01619-2, Human SHC1 Recombinant Matched Antibody Pair - PBS only. 84853-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3165. 84853-2-PBS was HRP conjugated as the detection antibody. Range: 125-4000 pg/mL.

    Sandwich ELISA standard curve of MP01619-2

    Sandwich ELISA standard curve of MP01619-2, Human SHC1 Recombinant Matched Antibody Pair - PBS only. 84853-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3165. 84853-2-PBS was HRP conjugated as the detection antibody. Range: 125-4000 pg/mL.

  • The mean SHC1 concentration was determined to be 18.0 ng/mL in Jurkat cell extract based on a 1.5 mg/mL extract load.

    Sample test of MP01619-2

    The mean SHC1 concentration was determined to be 18.0 ng/mL in Jurkat cell extract based on a 1.5 mg/mL extract load.

  • Cytometric bead array standard curve of MP01619-2, SHC1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84853-3-PBS. Detection antibody: 84853-2-PBS. Standard: Ag3165. Range: 0.156-20 ng/mL.

    Cytometric bead array standard curve of MP01619-2

    Cytometric bead array standard curve of MP01619-2, SHC1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84853-3-PBS. Detection antibody: 84853-2-PBS. Standard: Ag3165. Range: 0.156-20 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP01619-2 targets SHC1 in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.156-20 ng/mL (Cytometric Bead Array); 125-4000 pg/mL (Sandwich ELISA);

Capture antibody: SHC1 Recombinant antibody, PBS Only (Capture) 84853-3-PBS (242331H1). 100 μg. Concentration 1 mgl/ml.

Detection antibody: SHC1 Recombinant antibody, PBS Only (Detector) 84853-2-PBS (242331D5). 100 μg. Concentration 1 mgl/ml.

Alternative SHC1 matched antibody pairs: MP01619-1, MP50763-1, MP50763-2, MP50763-3, MP50763-4

Matched pair protein standard: Ag3165

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 84853-3-PBS
Tested Reactivity human
Immunogen SHC1 fusion protein Ag3165
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name SHC (Src homology 2 domain containing) transforming protein 1
Synonyms SHC, SHC-transforming protein 3, SHC-transforming protein 1, SHC transforming protein 1, SH2 domain protein C1
GenBank accession numberBC014158
Gene symbol SHC1
UniprotP29353
Gene ID (NCBI) 6464
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 84853-2-PBS
Tested Reactivity human
Immunogen SHC1 fusion protein Ag3165
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name SHC (Src homology 2 domain containing) transforming protein 1
Synonyms SHC, SHC-transforming protein 3, SHC-transforming protein 1, SHC transforming protein 1, SH2 domain protein C1
GenBank accession numberBC014158
Gene symbol SHC1
UniprotP29353
Gene ID (NCBI) 6464
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.