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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SHCBP1 Monoclonal antibody, PBS Only

SHCBP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B2E12

Cat no : 68647-1-PBS

Synonyms

SHC SH2 domain-binding protein 1, PAL

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SHCBP1 Monoclonal antibody (68647-1-Ig)

    WB analysis using 68647-1-Ig (same clone as 68647-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68647-1-Ig (SHCBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SHCBP1 Monoclonal antibody (68647-1-Ig)

    IHC staining of human liver cancer using 68647-1-Ig (same clone as 68647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68647-1-Ig (SHCBP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SHCBP1 Monoclonal antibody (68647-1-Ig)

    IHC staining of human liver cancer using 68647-1-Ig (same clone as 68647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68647-1-Ig (SHCBP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SHCBP1 Monoclonal antibody (68647-1-Ig)

    IHC staining of human breast cancer using 68647-1-Ig (same clone as 68647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68647-1-Ig (SHCBP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SHCBP1 Monoclonal antibody (68647-1-Ig)

    IHC staining of human breast cancer using 68647-1-Ig (same clone as 68647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68647-1-Ig (SHCBP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68647-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SHCBP1 Monoclonal antibody (68647-1-Ig)

    IF Staining of HEK-293 using 68647-1-Ig (same clone as 68647-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SHCBP1 antibody (68647-1-Ig, Clone: 1B2E12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68647-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68647-1-PBS targets SHCBP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SHCBP1 fusion protein Ag17517 相同性解析による交差性が予測される生物種
Full Name SHC SH2-domain binding protein 1
Calculated molecular weight 672 aa, 76 kDa
Observed molecular weight76 kDa
GenBank accession numberBC030699
Gene symbol SHCBP1
Gene ID (NCBI) 79801
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SHCBP1 was initially identified as a potential gene associated with cell proliferation in fibroblasts. SHCBP1 exerts a crucial physiological function in normal tissue development. Chen et al. showed that SHCBP1 is an essential component of FGF signaling in neural progenitor cells. SHCBP1 is also upregulated during T cell proliferation and regulates CD4+ T cell effector function in vivo. Recently, SHCBP1 was shown to be closely associated with cancer development.