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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SHD Polyclonal antibody

SHD Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20814-1-AP

Synonyms

SHD

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using SHD Polyclonal antibody (20814-1-AP)

    WB analysis of mouse brain using 20814-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20814-1-AP (SHD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SMMC-7721 cells using SHD Polyclonal antibody (20814-1-AP)

    WB analysis of SMMC-7721 using 20814-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 20814-1-AP (SHD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using SHD Polyclonal antibody (20814-1-AP)

    WB analysis of NIH/3T3 using 20814-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20814-1-AP (SHD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Neuro-2a cells using SHD Polyclonal antibody (20814-1-AP)

    WB analysis of Neuro-2a using 20814-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 20814-1-AP (SHD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SHD Polyclonal antibody (20814-1-AP)

    WB analysis of mouse brain using 20814-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20814-1-AP (SHD Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using SHD Polyclonal antibody (20814-1-AP)

    IF Staining of Neuro-2a using 20814-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed Neuro-2a cells using 20814-1-AP (SHD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse brain tissue, Neuro-2a cells, NIH/3T3 cells, SMMC-7721 cells
Positive IF/ICC detected inNeuro-2a cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20814-1-AP is SHD Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHD fusion protein Ag14794 相同性解析による交差性が予測される生物種
Full Name Src homology 2 domain containing transforming protein D
Calculated molecular weight 340 aa, 38 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberBC007206
Gene symbol SHD
Gene ID (NCBI) 56961
RRIDAB_2878745
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SHD antibody 20814-1-APDownload protocol
IF protocol for SHD antibody 20814-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols