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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SHE Polyclonal antibody

SHE Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22902-1-AP

Synonyms

RP11 350G8.8, SHE

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using SHE Polyclonal antibody (22902-1-AP)

    WB analysis of mouse testis using 22902-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 22902-1-AP (SHE Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using SHE Polyclonal antibody (22902-1-AP)

    WB analysis of mouse testis using 22902-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 22902-1-AP (SHE Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SHE Polyclonal antibody (22902-1-AP)

    IHC staining of human breast cancer using 22902-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22902-1-AP (SHE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SHE Polyclonal antibody (22902-1-AP)

    IHC staining of human breast cancer using 22902-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22902-1-AP (SHE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SHE Polyclonal antibody (22902-1-AP)

    IF Staining of HepG2 using 22902-1-AP

    Immunofluorescent analysis of HepG2 cells using 22902-1-AP (SHE antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22902-1-AP targets SHE in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name Src homology 2 domain containing E
Calculated molecular weight 54 kDa
Observed molecular weight 55 kDa
GenBank accession numberNM_001010846
Gene symbol SHE
Gene ID (NCBI) 126669
RRIDAB_2879185
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SHE, also known as SH2 domain-containing adapter protein E, is a 495 amino acid protein whose molecular weight is 54 kDa. The function of SHE remains unknown. This antibody specifically recognises the 54 kDa protein.

Protocols

Product Specific Protocols
WB protocol for SHE antibody 22902-1-APDownload protocol
IHC protocol for SHE antibody 22902-1-APDownload protocol
IF protocol for SHE antibody 22902-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols