Validation Data Gallery
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Tested Applications
| Positive IHC detected in | human skin cancer tissue, human cervical cancer tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 7 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
Product Information
13639-1-AP targets DSS1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | DSS1 fusion protein Ag4573 相同性解析による交差性が予測される生物種 |
| Full Name | split hand/foot malformation (ectrodactyly) type 1 |
| Calculated molecular weight | 70 aa, 8 kDa |
| GenBank accession number | BC032782 |
| Gene Symbol | DSS1 |
| Gene ID (NCBI) | 7979 |
| RRID | AB_2254633 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P60896 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DSS1 antibody 13639-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nature BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2.
| ||
EMBO J The proteasomal de-ubiquitinating enzyme POH1 promotes the double-strand DNA break response. | ||
J Med Genet Absent expression of the osteoblast-specific maternally imprinted genes, DLX5 and DLX6, causes split hand/split foot malformation type I. | ||
Lab Invest Increased chemosensitivity via BRCA2-independent DNA damage in DSS1- and PCID2-depleted breast carcinomas.
| ||
BMC Cancer Breast cancers with high DSS1 expression that potentially maintains BRCA2 stability have poor prognosis in the relapse-free survival. | ||
Protein Cell DSSylation, a novel protein modification targets proteins induced by oxidative stress, and facilitates their degradation in cells. |