Validation Data Gallery

Filter: AllWBIPIHCIFFC (Intra)

WB analysis using 20697-1-AP (same clone as 20697-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 20697-1-AP (SHH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20697-1-PBS in a different storage buffer formulation.
WB analysis using 20697-1-AP (same clone as 20697-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 20697-1-AP (SHH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20697-1-PBS in a different storage buffer formulation.
WB analysis of rat kidney using 20697-1-AP (same clone as 20697-1-PBS)
rat kidney tissue were subjected to SDS PAGE followed by western blot with 20697-1-AP (SHH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20697-1-PBS in a different storage buffer formulation.
IP experiment of mouse liver using 20697-1-AP (same clone as 20697-1-PBS)
IP result of anti-SHH (IP:20697-1-AP, 4ug; Detection:20697-1-AP 1:500) with mouse liver tissue lysate 6400ug. This data was developed using the same antibody clone with 20697-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 20697-1-AP (same clone as 20697-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20697-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 20697-1-AP (same clone as 20697-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20697-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 20697-1-AP (same clone as 20697-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20697-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 20697-1-AP (same clone as 20697-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20697-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 20697-1-AP (same clone as 20697-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20697-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 20697-1-AP (same clone as 20697-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20697-1-PBS in a different storage buffer formulation.
IHC staining of mouse embryo using 20697-1-AP (same clone as 20697-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20697-1-PBS in a different storage buffer formulation.
IHC staining of mouse embryo using 20697-1-AP (same clone as 20697-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20697-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 20697-1-AP (same clone as 20697-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20697-1-AP (SHH Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20697-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 20697-1-AP (same clone as 20697-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20697-1-AP (SHH Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20697-1-PBS in a different storage buffer formulation.
IF Staining of mouse kidney using 20697-1-AP (same clone as 20697-1-PBS)
Frozen tissue section of adult mouse kidney was stained for acetylated ɑ-tubulin (magenta, Cat. No CL488-66200), CD31/PECAM-1 (white), and Shh (green, Cat. No 20697-1-AP) with DAPI as a counterstain for visualizing the nucleus (blue). acetylated ɑ-tubulin stains primary cilia and was conjugated to Coralite-488 fluorescent dye and psedocolored to magenta. CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody and psedocolored to white. Shh stains Sonic Hedgehog and was visualized with an Alexa Fluor 555 secondary antibody and psedocolored to green.The Image and figure legends are intellectual property of @LAF_in_the_LAB. This data was developed using the same antibody clone with 20697-1-PBS in a different storage buffer formulation.
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Frozen tissue section of adult mouse kidney was stained for acetylated ɑ-tubulin (magenta, Cat. No CL488-66200), CD31/PECAM-1 (white), and Shh (green, Cat. No 20697-1-AP) with DAPI as a counterstain for visualizing the nucleus (blue). acetylated ɑ-tubulin stains primary cilia and was conjugated to Coralite-488 fluorescent dye and psedocolored to magenta. CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody and psedocolored to white. Shh stains Sonic Hedgehog and was visualized with an Alexa Fluor 555 secondary antibody and psedocolored to green.The Image and figure legends are intellectual property of @LAF_in_the_LAB. This data was developed using the same antibody clone with 20697-1-PBS in a different storage buffer formulation.
FC experiment of HepG2 using 20697-1-AP (same clone as 20697-1-PBS)
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human SHH (20697-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20697-1-PBS in a different storage buffer formulation.
×
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human SHH (20697-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20697-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

SHH Polyclonal antibody, PBS Only

SHH Polyclonal Antibody for WB, IHC, IF, FC (Intra), IP, Indirect ELISA

Cat No. 20697-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC (Intra), IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20697-1-PBS targets SHH in WB, IHC, IF, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	sonic hedgehog homolog (Drosophila)
Calculated molecular weight	50 kDa
Observed molecular weight	45-51 kDa
GenBank accession number	NM_000193
Gene Symbol	SHH
Gene ID (NCBI)	6469
RRID	AB_10694828
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q15465
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

Sonic hedgehog (SHH) is a member of the Hedgehog (Hh) family of proteins that control cell growth, survival,

and fate.

What is the molecular weight of SHH?

The molecular weight of SHH is approximately 45 kDa, but it is cleaved autocatalytically to generate a 20 kDa

N-terminal fragment and a 25 kDa C-terminal fragment responsible for hedgehog biological activity and the

auto-processing machinery respectively (PMID: 10753901).

What is the tissue specificity of SHH?

SHH is the most broadly expressed mammalian Hh signaling molecule. During early vertebrate development,

SHH is expressed in midline tissues such as the node, notochord, and floor plate to control the left-right and

dorso-ventral patterning of the embryo (PMID: 12729563). SHH is expressed in fetal intestine, liver, kidney,

and lung tissue, but is not expressed in adult tissues.

What is the cellular localization of SHH?

SHH is localized to the cell membrane. The N-terminal fragment is associated with lipid rafts at the cell surface,

whereas the C-terminal fragment diffuses from the cell.

What is the role of SHH in the Sonic hedgehog signaling pathway?

This pathway is one of the major trafficking networks that regulate key events during developmental growth

and patterning (PMID: 19368798). Loss of SHH signaling and regulation has been implicated in birth defects,

tissue regeneration, stem cell renewal, and cancer growth (PMID: 15549094). The regulatory action of the SHH

pathway is linked to the secretion, uptake, and translocation of the SHH protein; a ligand precursor, which is

implicated in the regulation of the central nervous system polarity and neural patterning (PMID: 11130178,

11801325).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 20697-1-AP (same clone as 20697-1-PBS)

WB analysis using 20697-1-AP (same clone as 20697-1-PBS)

WB analysis of rat kidney using 20697-1-AP (same clone as 20697-1-PBS)

IP experiment of mouse liver using 20697-1-AP (same clone as 20697-1-PBS)

IHC staining of human liver cancer using 20697-1-AP (same clone as 20697-1-PBS)

IHC staining of human liver cancer using 20697-1-AP (same clone as 20697-1-PBS)

IHC staining of human ovary tumor using 20697-1-AP (same clone as 20697-1-PBS)

IHC staining of human ovary tumor using 20697-1-AP (same clone as 20697-1-PBS)

IHC staining of human pancreas cancer using 20697-1-AP (same clone as 20697-1-PBS)