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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Mouse SHH Recombinant antibody, PBS Only

SHH Recombinant Antibody for Indirect ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

240057E7

Cat no : 83280-1-PBS

Synonyms

M100081, Hxl3, Hx, Hhg1, Dsh

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Shh Recombinant antibody (83280-1-RR)

    WB analysis using 83280-1-RR (same clone as 83280-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83280-1-RR (SHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83280-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83280-1-RR

    Affinity and Kinetic Characterization of 83280-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83280-1-RR against Mouse SHH were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83280-1-PBS is SHH Fusion Protein expressed in E. coli.

Tested Reactivity rat, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name sonic hedgehog
Calculated molecular weight48KD
GenBank accession numberNM_009170.3
Gene symbol Shh
Gene ID (NCBI) 20423
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.