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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SHKBP1 Polyclonal antibody

SHKBP1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12703-1-AP

Synonyms

SH3KBP1-binding protein 1, SH3KBP1 binding protein 1, SETA-binding protein 1, SB1, PP203

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using SHKBP1 Polyclonal antibody (12703-1-AP)

    WB analysis of A431 using 12703-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 12703-1-AP (SHKBP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SHKBP1 Polyclonal antibody (12703-1-AP)

    IF Staining of HepG2 using 12703-1-AP

    Immunofluorescent analysis of HepG2 cells, using SHKBP1 antibody 12703-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inA431 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12703-1-AP targets SHKBP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHKBP1 fusion protein Ag3386 相同性解析による交差性が予測される生物種
Full Name SH3KBP1 binding protein 1
Calculated molecular weight 707 aa, 76 kDa
Observed molecular weight 76 kDa
GenBank accession numberBC022855
Gene symbol SHKBP1
Gene ID (NCBI) 92799
RRIDAB_10637860
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SHKBP1 antibody 12703-1-APDownload protocol
IF protocol for SHKBP1 antibody 12703-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols