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- KD/KO Validated
SHMT2 Polyclonal antibody
SHMT2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 11099-1-AP
Synonyms
Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | HEK-293 cells, mouse kidney tissue, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells |
| Positive IHC detected in | human cervical cancer tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 22 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
Product Information
11099-1-AP targets SHMT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SHMT2 fusion protein Ag1584 相同性解析による交差性が予測される生物種 |
| Full Name | serine hydroxymethyltransferase 2 (mitochondrial) |
| Calculated molecular weight | 55 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC011911 |
| Gene symbol | SHMT2 |
| Gene ID (NCBI) | 6472 |
| RRID | AB_2188452 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SHMT2 antibody 11099-1-AP | Download protocol |
| IHC protocol for SHMT2 antibody 11099-1-AP | Download protocol |
| IF protocol for SHMT2 antibody 11099-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Pineal Res Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis. | ||
Nat Commun Defective NADPH production in mitochondrial disease complex I causes inflammation and cell death. | ||
Cell Res cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions. | ||
Am J Respir Cell Mol Biol Inhibition of PHGDH Attenuates Bleomycin-induced Pulmonary Fibrosis. | ||
PLoS Pathog SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.
| ||
Genomics SHMT2 promotes the tumorigenesis of renal cell carcinoma by regulating the m6A modification of PPAT. |
