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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SHPK Polyclonal antibody (12441-1-AP)

    WB analysis using 12441-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12441-1-AP (SHPK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SHPK Polyclonal antibody (12441-1-AP)

    WB analysis using 12441-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12441-1-AP (SHPK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SHPK Polyclonal antibody (12441-1-AP)

    IHC staining of human prostate cancer using 12441-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12441-1-AP (SHPK antibody) at dilution of 1:50 (under 10x lens).

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SHPK Polyclonal antibody

SHPK Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12441-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CARKL, sedoheptulokinase, SHK, SHPK

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMDA-MB-231 cells, RAW 264.7 cells, mouse heart tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12441-1-AP targets SHPK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHPK fusion protein Ag3150 相同性解析による交差性が予測される生物種
Full Name sedoheptulokinase
Calculated molecular weight 478 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC020543
Gene Symbol SHPK
Gene ID (NCBI) 23729
RRIDAB_2188566
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHJ6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SHPK, also named CARKL, is a carbohydrate kinase involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane (PubMed: 10673275). SHPK is a sedoheptulose kinase of the pentose phosphate pathway. It acts as a modulator of macrophage activation through control of glucose metabolism (PMID: 22682222).

Protocols

Product Specific Protocols
WB protocol for SHPK antibody 12441-1-APDownload protocol
IHC protocol for SHPK antibody 12441-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols