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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using SHPK Recombinant antibody (82875-1-RR)

    IF Staining of U2OS using 82875-1-RR (same clone as 82875-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using SHPK antibody (82875-1-RR, Clone: 230115A4 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82875-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-2 OS using SHPK Recombinant antibody (82875-1-RR)

    FC experiment of U-2 OS using 82875-1-RR (same clone as 82875-1-PBS)

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug Anti-Human SHPK (82875-1-RR, Clone:230115A4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82875-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82875-1-RR

    Affinity and Kinetic Characterization of 82875-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82875-1-RR against Human SHPK were performed. The affinity constant is 18.1 pM.

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SHPK Recombinant antibody, PBS Only

SHPK Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82875-1-PBS
Clone No.230115A4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

SHK, Sedoheptulokinase, EC:2.7.1.14, CARKL, Carbohydrate kinase-like protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82875-1-PBS targets SHPK in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SHPK fusion protein Ag33893 相同性解析による交差性が予測される生物種
Full Name sedoheptulokinase
Calculated molecular weight 478 aa, 52 kDa
GenBank accession numberBC020543
Gene Symbol SHPK
Gene ID (NCBI) 23729
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UHJ6
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

SHPK, also named CARKL, is a carbohydrate kinase involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane (PubMed: 10673275). SHPK is a sedoheptulose kinase of the pentose phosphate pathway. It acts as a modulator of macrophage activation through control of glucose metabolism (PMID: 22682222).