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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SHROOM1 Polyclonal antibody

SHROOM1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 18218-1-AP

Synonyms

Apical protein 2, APXL2, KIAA1960, Protein Shroom1, shroom family member 1, SHROOM1

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using SHROOM1 Polyclonal antibody (18218-1-AP)

    WB analysis of MCF-7 using 18218-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 18218-1-AP (SHROOM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using SHROOM1 Polyclonal antibody (18218-1-AP)

    WB analysis of A375 using 18218-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 18218-1-AP (SHROOM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using SHROOM1 Polyclonal antibody (18218-1-AP)

    IP experiment of MCF-7 using 18218-1-AP

    IP result of anti-SHROOM1 (IP:18218-1-AP, 4ug; Detection:18218-1-AP 1:700) with MCF-7 cells lysate 3600 ug.

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Tested Applications

Positive WB detected inMCF7 cells, A375 cells
Positive IP detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 18218-1-AP is SHROOM1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHROOM1 fusion protein Ag12977 相同性解析による交差性が予測される生物種
Full Name shroom family member 1
Calculated molecular weight 852 aa, 91 kDa
Observed molecular weight 100-130 kDa
GenBank accession numberBC104914
Gene symbol SHROOM1
Gene ID (NCBI) 134549
RRIDAB_10858616
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SHROOM1 antibody 18218-1-APDownload protocol
IP protocol for SHROOM1 antibody 18218-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Pathog

Defining the proteolytic landscape during enterovirus infection.

Authors - Mohsan Saeed
View Article