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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SIAE Polyclonal antibody

SIAE Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14566-1-AP

Synonyms

Sialate O-acetylesterase, LSE, H-Lse, H Lse, EC:3.1.1.53

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SIAE Polyclonal antibody (14566-1-AP)

    WB analysis using 14566-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14566-1-AP (SIAE antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse testis tissue, rat heart tissue, rat testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14566-1-AP targets SIAE in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIAE fusion protein Ag6143 相同性解析による交差性が予測される生物種
Full Name sialic acid acetylesterase
Calculated molecular weight 58 kDa
Observed molecular weight62-70 kDa
GenBank accession numberBC068450
Gene symbol SIAE
Gene ID (NCBI) 54414
RRIDAB_3669189
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sialic acids as terminal sugars of N- and O-glycosylated macromolecules are decisive for the functions of glycoconjugates both in soluble form and integrated into cell membranes .The two enzymes responsible for acetylation and de-acetylation have been described as sialate O-acetyltransferase (SOAT) and sialate O-acetylesterase (SIAE), respectively. SIAE deacetylates α(2-6)-linked sialic acid on N-linked glycans of BCR, allowing the interaction with CD22, that functions in vivo as an inhibitor of BCR signaling. (PMID: 26022516)

Protocols

Product Specific Protocols
WB protocol for SIAE antibody 14566-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols