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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SIAE Monoclonal antibody (66522-1-Ig)

    WB analysis using 66522-1-Ig (same clone as 66522-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66522-1-Ig (SIAE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66522-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig colon tissue using SIAE Monoclonal antibody (66522-1-Ig)

    WB analysis of pig colon using 66522-1-Ig (same clone as 66522-1-PBS)

    pig colon tissue were subjected to SDS PAGE followed by western blot with 66522-1-Ig (SIAE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66522-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using SIAE Monoclonal antibody (66522-1-Ig)

    WB analysis of pig liver using 66522-1-Ig (same clone as 66522-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66522-1-Ig (SIAE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66522-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using SIAE Monoclonal antibody (66522-1-Ig)

    IF Staining of PC-3 using 66522-1-Ig (same clone as 66522-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using SIAE antibody (66522-1-Ig, Clone: 1G5F7 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66522-1-PBS in a different storage buffer formulation.

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SIAE Monoclonal antibody, PBS Only

SIAE Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66522-1-PBS
Clone No.1G5F7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Sialate O-acetylesterase, LSE, H-Lse, H Lse, EC:3.1.1.53

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66522-1-PBS targets SIAE in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SIAE fusion protein Ag14624 相同性解析による交差性が予測される生物種
Full Name sialic acid acetylesterase
Calculated molecular weight 58 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC068450
Gene Symbol SIAE
Gene ID (NCBI) 54414
RRIDAB_2881885
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9HAT2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.