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KD/KO Validated

SIAH1 Polyclonal antibody

Name: SIAH1 Polyclonal antibody
Brand: Proteintech
SKU: 13886-1-AP

SIAH1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13886-1-AP

Synonyms

hSIAH1, HUMSIAH, Seven in absentia homolog 1, Siah 1, Siah 1a, SIAH1

Validation Data Gallery

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AllWBIHC

WB analysis of HeLa using 13886-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 13886-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 13886-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of RAW264.7 using 13886-1-AP
RAW264.7 cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse small intestine using 13886-1-AP
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 13886-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 13886-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 13886-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 13886-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13886-1-AP (SIAH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 13886-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13886-1-AP (SIAH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 13886-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, A549 cells, COLO 320 cells, HepG2 cells, Jurkat cells, mouse small intestine tissue
Positive IHC detected in	mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below

Product Information

13886-1-AP targets SIAH1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SIAH1 fusion protein Ag4336 相同性解析による交差性が予測される生物種
Full Name	seven in absentia homolog 1 (Drosophila)
Calculated molecular weight	282 aa, 31 kDa
Observed molecular weight	60 kDa
GenBank accession number	BC035562
Gene symbol	SIAH1
Gene ID (NCBI)	6477
RRID	AB_10643374
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SIAH1, also named as HUMSIAH, Siah-1a and Siah-1S(21kd), belongs to the SINA (Seven in absentia) family. SIAH1 is known to cause indirect degradation of beta-catenin through formation of a complex with Siah-interacting protein (SIP), Skp1 and Ebi. SIAH1 can form heterodimer or homodimer such as Siah-1*Siah-1(70 or 62kd), Siah-1*Siah-1S(42kd) and Siah-1S*Siah-1S(51-56kd). Importantly, results from in vitro soft agar assay demonstrated that Siah-1S displays a promotion effect on cells tumorigenicity. (PMID:17420721)

Protocols

Product Specific Protocols
WB protocol for SIAH1 antibody 13886-1-AP	Download protocol
IHC protocol for SIAH1 antibody 13886-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Rep Regulation of EZH2 by SMYD2-Mediated Lysine Methylation Is Implicated in Tumorigenesis. Authors - Yi Zeng View Article
human	WB	Exp Cell Res Siah1 promotes the proliferation of NSCLC cells through ubiquitinating and stabilizing Notch1 Authors - Yan Liu KD Validated View Article
human	WB	iScience Cathepsin K promotes the proliferation of hepatocellular carcinoma cells through induction of SIAH1 ubiquitination and degradation Authors - Chengming Zhang View Article
human	WB	J Exp Clin Cancer Res GABA induced by sleep deprivation promotes the proliferation and migration of colon tumors through miR-223-3p endogenous pathway and exosome pathway Authors - Haijun Bao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SIAH1 Polyclonal antibody

SIAH1 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of HeLa using 13886-1-AP

WB analysis of A549 using 13886-1-AP

WB analysis of Jurkat using 13886-1-AP

WB analysis of RAW264.7 using 13886-1-AP

WB analysis of mouse small intestine using 13886-1-AP

WB analysis of HeLa using 13886-1-AP

WB analysis of COLO 320 using 13886-1-AP

WB analysis of HepG2 using 13886-1-AP

IHC staining of mouse testis using 13886-1-AP

IHC staining of mouse testis using 13886-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Regulation of EZH2 by SMYD2-Mediated Lysine Methylation Is Implicated in Tumorigenesis.

Siah1 promotes the proliferation of NSCLC cells through ubiquitinating and stabilizing Notch1

Cathepsin K promotes the proliferation of hepatocellular carcinoma cells through induction of SIAH1 ubiquitination and degradation

GABA induced by sleep deprivation promotes the proliferation and migration of colon tumors through miR-223-3p endogenous pathway and exosome pathway