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Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using SIKE1 Polyclonal antibody (22432-1-AP)

    WB analysis of SH-SY5Y using 22432-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22432-1-AP (SIKE1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using SIKE1 Polyclonal antibody (22432-1-AP)

    WB analysis of MCF-7 using 22432-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 22432-1-AP (SIKE1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SIKE1 Polyclonal antibody (22432-1-AP)

    WB analysis of mouse brain using 22432-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22432-1-AP (SIKE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SIKE1 Polyclonal antibody (22432-1-AP)

    WB analysis of HEK-293 using 22432-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 22432-1-AP (SIKE1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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SIKE1 Polyclonal antibody

SIKE1 Polyclonal Antibody for WB, ELISA
Cat No. 22432-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Suppressor of IKK-epsilon, suppressor of IKK epsilon, Suppressor of IKBKE 1, SIKE, RP5-1000E10.4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSH-SY5Y cells, HEK-293 cells, MCF-7 cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22432-1-AP targets SIKE1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIKE1 fusion protein Ag18196 相同性解析による交差性が予測される生物種
Full Name suppressor of IKK epsilon
Calculated molecular weight 207 aa, 24 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC005934
Gene Symbol SIKE1
Gene ID (NCBI) 80143
RRIDAB_10914502
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRV8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SIKE1 antibody 22432-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols