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Species Reactivity

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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SIL1 Polyclonal antibody (24110-1-AP)

    WB analysis using 24110-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24110-1-AP (SIL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using SIL1 Polyclonal antibody (24110-1-AP)

    WB analysis of mouse kidney using 24110-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24110-1-AP (SIL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SIL1 Polyclonal antibody (24110-1-AP)

    IHC staining of human kidney using 24110-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24110-1-AP (SIL1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SIL1 Polyclonal antibody (24110-1-AP)

    IHC staining of human kidney using 24110-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24110-1-AP (SIL1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using SIL1 Polyclonal antibody (24110-1-AP)

    IHC staining of mouse colon using 24110-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24110-1-AP (SIL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using SIL1 Polyclonal antibody (24110-1-AP)

    IHC staining of mouse colon using 24110-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24110-1-AP (SIL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using SIL1 Polyclonal antibody (24110-1-AP)

    IHC staining of mouse colon using 24110-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24110-1-AP (SIL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SIL1 Polyclonal antibody (24110-1-AP)

    IHC staining of mouse testis using 24110-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24110-1-AP (SIL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using SIL1 Polyclonal antibody (24110-1-AP)

    IHC staining of mouse testis using 24110-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24110-1-AP (SIL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SIL1 Polyclonal antibody (24110-1-AP)

    IF Staining of HepG2 using 24110-1-AP

    Immunofluorescent analysis of HepG2 cells using 24110-1-AP (SIL1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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SIL1 Polyclonal antibody

SIL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24110-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

UNQ545/PRO836, ULG5, MSS, BiP-associated protein, BiP associated protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inL02 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman kidney tissue, mouse colon tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

24110-1-AP targets SIL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIL1 fusion protein Ag21367 相同性解析による交差性が予測される生物種
Full Name SIL1 homolog, endoplasmic reticulum chaperone (S. cerevisiae)
Calculated molecular weight 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC011568
Gene Symbol SIL1
Gene ID (NCBI) 64374
RRIDAB_2879434
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9H173
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SIL1 antibody 24110-1-APDownload protocol
IHC protocol for SIL1 antibody 24110-1-APDownload protocol
IF protocol for SIL1 antibody 24110-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Sci

Proteomic Analysis of Marinesco-Sjogren Syndrome Fibroblasts Indicates Pro-Survival Metabolic Adaptation to SIL1 Loss.

Authors - Francesca Potenza
View Article
mouseWB

Mol Biol Cell

Sil1, a nucleotide exchange factor for BiP, is not required for antibody assembly or secretion.

Authors - Viraj P Ichhaporia
View Article
mouseWB

Mol Neurobiol

Loss of SIL1 Affects Actin Dynamics and Leads to Abnormal Neural Migration

Authors - Yuanyuan Xu
View Article