SIN3A Polyclonal antibody

SIN3A Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14638-1-AP

Synonyms

KIAA0700


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Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells, K-562 cells
Positive IP detected inJurkat cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14638-1-AP targets SIN3A in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIN3A fusion protein Ag6219 相同性解析による交差性が予測される生物種
Full Name SIN3 homolog A, transcription regulator (yeast)
Calculated molecular weight 145 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC066364
Gene symbol SIN3A
Gene ID (NCBI) 25942
RRIDAB_2301741
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SIN3A antibody 14638-1-APDownload protocol
IF protocol for SIN3A antibody 14638-1-APDownload protocol
IP protocol for SIN3A antibody 14638-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Theranostics

SLC14A1 prevents oncometabolite accumulation and recruits HDAC1 to transrepress oncometabolite genes in urothelial carcinoma.

Authors - Ti-Chun Chan
humanWB,CoIP

Int J Biol Sci

TET1s deficiency exacerbates oscillatory shear flow-induced atherosclerosis.

Authors - Kai Qu

Cell Rep

ZNF451 favors triple-negative breast cancer progression by enhancing SLUG-mediated CCL5 transcriptional expression

Authors - Yu Zhang
mouseWB,IP

Nat Commun

Glucocorticoids increase adiposity by stimulating Krüppel-like factor 9 expression in macrophages

Authors - Yinliang Zhang
ratWB

Bioact Mater

Chitosan/PLGA-based tissue engineered nerve grafts with SKP-SC-EVs enhance sciatic nerve regeneration in dogs through miR-30b-5p-mediated regulation of axon growth

Authors - Miaomei Yu