Validation Data Gallery

Filter: AllWBIPIF/ICC

WB analysis of Jurkat using 14638-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 14638-1-AP (SIN3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14638-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14638-1-AP (SIN3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 14638-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14638-1-AP (SIN3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 14638-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 14638-1-AP (SIN3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 14638-1-AP
IP result of anti-SIN3A (IP:14638-1-AP, 4ug; Detection:14638-1-AP 1:500) with Jurkat cells lysate 3600ug.
IF Staining of HepG2 using 14638-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SIN3A antibody (14638-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

SIN3A Polyclonal antibody

SIN3A Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 14638-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, CoIP, ELISA

Synonyms

KIAA0700

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, HEK-293 cells, HeLa cells, K-562 cells
Positive IP detected in	Jurkat cells
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

14638-1-AP targets SIN3A in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SIN3A fusion protein Ag6219 相同性解析による交差性が予測される生物種
Full Name	SIN3 homolog A, transcription regulator (yeast)
Calculated molecular weight	145 kDa
Observed molecular weight	150 kDa
GenBank accession number	BC066364
Gene Symbol	SIN3A
Gene ID (NCBI)	25942
RRID	AB_2301741
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96ST3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for SIN3A antibody 14638-1-AP	Download protocol
IF protocol for SIN3A antibody 14638-1-AP	Download protocol
IP protocol for SIN3A antibody 14638-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Theranostics SLC14A1 prevents oncometabolite accumulation and recruits HDAC1 to transrepress oncometabolite genes in urothelial carcinoma. Authors - Ti-Chun Chan View Article
human	WB,CoIP	Int J Biol Sci TET1s deficiency exacerbates oscillatory shear flow-induced atherosclerosis. Authors - Kai Qu View Article
		Cell Rep ZNF451 favors triple-negative breast cancer progression by enhancing SLUG-mediated CCL5 transcriptional expression Authors - Yu Zhang View Article
mouse	WB,IP	Nat Commun Glucocorticoids increase adiposity by stimulating Krüppel-like factor 9 expression in macrophages Authors - Yinliang Zhang View Article
rat	WB	Bioact Mater Chitosan/PLGA-based tissue engineered nerve grafts with SKP-SC-EVs enhance sciatic nerve regeneration in dogs through miR-30b-5p-mediated regulation of axon growth Authors - Miaomei Yu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Jurkat using 14638-1-AP

WB analysis of HeLa using 14638-1-AP

WB analysis of HEK-293 using 14638-1-AP

WB analysis of K-562 using 14638-1-AP

IP experiment of Jurkat using 14638-1-AP

IF Staining of HepG2 using 14638-1-AP

SIN3A Polyclonal antibody

SIN3A Polyclonal Antibody for WB, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

SLC14A1 prevents oncometabolite accumulation and recruits HDAC1 to transrepress oncometabolite genes in urothelial carcinoma.

TET1s deficiency exacerbates oscillatory shear flow-induced atherosclerosis.

ZNF451 favors triple-negative breast cancer progression by enhancing SLUG-mediated CCL5 transcriptional expression

Glucocorticoids increase adiposity by stimulating Krüppel-like factor 9 expression in macrophages

Chitosan/PLGA-based tissue engineered nerve grafts with SKP-SC-EVs enhance sciatic nerve regeneration in dogs through miR-30b-5p-mediated regulation of axon growth