Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells, rat brain tissue |
| Positive IP detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
21672-1-AP targets SIP1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SIP1 fusion protein Ag16500 相同性解析による交差性が予測される生物種 |
| Full Name | survival of motor neuron protein interacting protein 1 |
| Calculated molecular weight | 280 aa, 32 kDa |
| Observed molecular weight | 32-35 kDa |
| GenBank accession number | BC104968 |
| Gene Symbol | SIP1 |
| Gene ID (NCBI) | 8487 |
| RRID | AB_10859249 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14893 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SIP1 antibody 21672-1-AP | Download protocol |
| IP protocol for SIP1 antibody 21672-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Rep Functional interaction between FUS and SMN underlies SMA-like splicing changes in wild-type hFUS mice. | ||
Neurobiol Dis UsnRNP trafficking is regulated by stress granules and compromised by mutant ALS proteins. | ||
J Mol Med (Berl) Hyper-SUMOylation of SMN induced by SENP2 deficiency decreases its stability and leads to spinal muscular atrophy-like pathology. | ||
Biomolecules (20S)G-Rh2 Inhibits NF-κB Regulated Epithelial-Mesenchymal Transition by Targeting Annexin A2. |