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Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using SIPA1 Polyclonal antibody (26793-1-AP)

    WB analysis of Raji using 26793-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 26793-1-AP (SIPA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293T cells using SIPA1 Polyclonal antibody (26793-1-AP)

    WB analysis of HEK-293T using 26793-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 26793-1-AP (SIPA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using SIPA1 Polyclonal antibody (26793-1-AP)

    IHC staining of human ovary cancer using 26793-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 26793-1-AP (SIPA1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SIPA1 Polyclonal antibody (26793-1-AP)

    IF Staining of MCF-7 using 26793-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SIPA1 antibody (26793-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SIPA1 Polyclonal antibody (26793-1-AP)

    IF Staining of MCF-7 using 26793-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using SIPA1 antibody (26793-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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SIPA1 Polyclonal antibody

SIPA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 26793-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Sipa-1, Sipa 1, Signal-induced proliferation-associated protein 1, p130 SPA-1, p130 SPA 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRaji cells, HEK-293T cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26793-1-AP targets SIPA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIPA1 fusion protein Ag25383 相同性解析による交差性が予測される生物種
Full Name signal-induced proliferation-associated 1
Calculated molecular weight 1042 aa, 112 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC010492
Gene Symbol SIPA1
Gene ID (NCBI) 6494
RRIDAB_2880637
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96FS4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SIPA1 (Signal-induced proliferation-associated protein 1) is also named as SPA1, p130 SPA-1 and GTPase-activating protein Spa-1. SIPA1, a GTPase activating protein, was discovered in proliferating lymphocytes as mitogen-induced nuclear protein. SIPA1 promotes catalyzation of hydrolyze Rap1GTP/GDP and is known to be a negative regulator of Ras-related protein, which transduces the signals for various cellular functions, including development, cellular proliferation, and cellular adhesion (PMID:28237246). Transcription of fibronectin 1, which is crucial for cell junction and migration of triple-negative breast cancer (TNBC) cells, was regulated by SIPA1 in a DBR-dependent manner. SIPA1 was highly expressed in metastatic TNBC. SIPA1 served as a TF, promoting TNBC migration, invasion, and metastasis (PMID: 37500797).

Protocols

Product Specific Protocols
WB protocol for SIPA1 antibody 26793-1-APDownload protocol
IHC protocol for SIPA1 antibody 26793-1-APDownload protocol
IF protocol for SIPA1 antibody 26793-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Transl Oncol

Identification of metastasis-associated exoDEPs in colorectal cancer using label-free proteomics.

Authors - Xinlu Liu
View Article