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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SIPA1L1 Polyclonal antibody

SIPA1L1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 25086-1-AP

Synonyms

SIPA1 like protein 1, Signal-induced proliferation-associated 1-like protein 1, High-risk human papilloma viruses E6 oncoproteins targeted protein 1, E6TP1, E6-targeted protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SIPA1L1 Polyclonal antibody (25086-1-AP)

    WB analysis using 25086-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25086-1-AP (SIPA1L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SIPA1L1 Polyclonal antibody (25086-1-AP)

    WB analysis of HeLa using 25086-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25086-1-AP (SIPA1L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using SIPA1L1 Polyclonal antibody (25086-1-AP)

    IP experiment of mouse brain using 25086-1-AP

    IP result of anti-SIPA1L1 (IP:25086-1-AP, 4ug; Detection:25086-1-AP 1:3000) with mouse brain tissue lysate 1280 ug.

  • Immunohistochemistry (IHC) staining of human brain tissue using SIPA1L1 Polyclonal antibody (25086-1-AP)

    IHC staining of human brain using 25086-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25086-1-AP (SIPA1L1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using SIPA1L1 Polyclonal antibody (25086-1-AP)

    IHC staining of human brain using 25086-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25086-1-AP (SIPA1L1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using SIPA1L1 Polyclonal antibody (25086-1-AP)

    IHC staining of human pancreas using 25086-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25086-1-AP (SIPA1L1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using SIPA1L1 Polyclonal antibody (25086-1-AP)

    IHC staining of human pancreas using 25086-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 25086-1-AP (SIPA1L1 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25086-1-AP targets SIPA1L1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIPA1L1 fusion protein Ag18900 相同性解析による交差性が予測される生物種
Full Name signal-induced proliferation-associated 1 like 1
Calculated molecular weight 1803 aa, 200 kDa
Observed molecular weight 200 kDa
GenBank accession numberBC146777
Gene symbol SIPA1L1
Gene ID (NCBI) 26037
RRIDAB_2714023
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SIPA1L1 antibody 25086-1-APDownload protocol
IHC protocol for SIPA1L1 antibody 25086-1-APDownload protocol
IP protocol for SIPA1L1 antibody 25086-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IP

Neuron

Prosapip1-Dependent Synaptic Adaptations in the Nucleus Accumbens Drive Alcohol Intake, Seeking, and Reward.

Authors - Sophie Laguesse
View Article
mouseWB

bioRxiv

Prosapip1 in the dorsal hippocampus mediates synaptic protein composition, long-term potentiation, and spatial memory

Authors - Zachary W Hoisington
View Article
humanWB,IF

JCI Insight

Effects of SIPA1L1 on trabecular meshwork extracellular matrix protein accumulation and cellular phagocytosis in POAG

Authors - Chenyu Xu
  • KD Validated
View Article