SIPA1L1 Polyclonal antibody
SIPA1L1 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 25086-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, HeLa cells, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human brain tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 3 publications below |
IF | See 1 publications below |
IP | See 1 publications below |
Product Information
25086-1-AP targets SIPA1L1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SIPA1L1 fusion protein Ag18900 相同性解析による交差性が予測される生物種 |
Full Name | signal-induced proliferation-associated 1 like 1 |
Calculated molecular weight | 1803 aa, 200 kDa |
Observed molecular weight | 200 kDa |
GenBank accession number | BC146777 |
Gene symbol | SIPA1L1 |
Gene ID (NCBI) | 26037 |
RRID | AB_2714023 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for SIPA1L1 antibody 25086-1-AP | Download protocol |
IHC protocol for SIPA1L1 antibody 25086-1-AP | Download protocol |
IP protocol for SIPA1L1 antibody 25086-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Neuron Prosapip1-Dependent Synaptic Adaptations in the Nucleus Accumbens Drive Alcohol Intake, Seeking, and Reward. | ||
bioRxiv Prosapip1 in the dorsal hippocampus mediates synaptic protein composition, long-term potentiation, and spatial memory | ||
JCI Insight Effects of SIPA1L1 on trabecular meshwork extracellular matrix protein accumulation and cellular phagocytosis in POAG
|