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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SIPA1L3 Polyclonal antibody (30544-1-AP)

    WB analysis using 30544-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30544-1-AP (SIPA1L3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SIPA1L3 Polyclonal antibody (30544-1-AP)

    WB analysis using 30544-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30544-1-AP (SIPA1L3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using SIPA1L3 Polyclonal antibody (30544-1-AP)

    IP experiment of HeLa using 30544-1-AP

    IP result of anti-SIPA1L3 (IP:30544-1-AP, 4ug; Detection:30544-1-AP 1:3000) with HeLa cells lysate 1320 ug.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using SIPA1L3 Polyclonal antibody (30544-1-AP)

    IHC staining of human ovary cancer using 30544-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30544-1-AP (SIPA1L3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SIPA1L3 Polyclonal antibody (30544-1-AP)

    IF Staining of HeLa using 30544-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SIPA1L3 antibody (30544-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SIPA1L3 Polyclonal antibody (30544-1-AP)

    IF Staining of HEK-293 using 30544-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SIPA1L3 antibody (30544-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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SIPA1L3 Polyclonal antibody

SIPA1L3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 30544-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

SPA-1-like protein 3, SPA 1 like protein 3, SIPA1-like protein 3, SIPA1 like protein 3, Signal-induced proliferation-associated 1-like protein 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30544-1-AP targets SIPA1L3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIPA1L3 fusion protein Ag30750 相同性解析による交差性が予測される生物種
Full Name signal-induced proliferation-associated 1 like 3
Observed molecular weight 195 kDa
GenBank accession numberBC150620
Gene Symbol SIPA1L3
Gene ID (NCBI) 23094
RRIDAB_3086357
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60292
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STEAP3 (Signal-induced proliferation-associated 1-like protein 3) is also named as KIAA0545 and SPAL3. STEAP3 is a member of the STEAP family and is composed of a six-transmembrane domain at the COOH-terminal domain and a cytoplasmic N-terminal oxidoreductase domain, which is essential for iron and copper uptake (PMID:16227996). STEAP3 contains a functional p53-binding site in its promoter and can be upregulated following p53 activation to enhance cell death in myeloid leukemia cell line and breast cancer cells (PMID: 18617898). By interacting with Nix, a pro-apoptotic Bcl-2 family member, and Myt1 kinase, a negative regulator of the G2/M transition, STEAP3 overexpression promotes apoptosis and inhibits G2/M transition in cell cycle progression (PMID: 12606722, PMID: 10504341).

Protocols

Product Specific Protocols
WB protocol for SIPA1L3 antibody 30544-1-APDownload protocol
IHC protocol for SIPA1L3 antibody 30544-1-APDownload protocol
IF protocol for SIPA1L3 antibody 30544-1-APDownload protocol
IP protocol for SIPA1L3 antibody 30544-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IP,IF

bioRxiv

Uncovering the electrical synapse proteome in retinal neurons via in vivo proximity labeling

Authors - Stephan Tetenborg
View Article