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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SIRP Alpha/CD172a Polyclonal antibody (31485-1-AP)

    WB analysis using 31485-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31485-1-AP (SIRP Alpha/CD172a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SIRP Alpha Polyclonal antibody (31485-1-AP)

    WB analysis using 31485-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 31485-1-AP (SIRP Alpha antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using SIRP Alpha/CD172a Polyclonal antibody (31485-1-AP)

    IF Staining of THP-1 using 31485-1-AP

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using SIRP Alpha/CD172a antibody (31485-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of THP-1 cells using SIRP Alpha/CD172a Polyclonal antibody (31485-1-AP)

    FC experiment of THP-1 using 31485-1-AP

    1x10^6 THP-1 cells were intracellularly stained with 0.25 ug SIRP Alpha/CD172a Polyclonal antibody (31485-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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SIRP Alpha/CD172a Polyclonal antibody

SIRP Alpha/CD172a Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 31485-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

SIRP Alpha, SIRPA, MFR, Inhibitory receptor SHPS-1, CD172a

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inC6 cells, NIH/3T3 cells, U-937 cells
Positive IF/ICC detected inTHP-1 cells
Positive FC (Intra) detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31485-1-AP targets SIRP Alpha/CD172a in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name signal-regulatory protein alpha
Calculated molecular weight55 kDa
Observed molecular weight65-100 kDa
GenBank accession numberNM_080792
Gene Symbol SIRP Alpha
Gene ID (NCBI) 140885
RRIDAB_3670002
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP78324
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).

Protocols

Product Specific Protocols
WB protocol for SIRP Alpha/CD172a antibody 31485-1-APDownload protocol
IF protocol for SIRP Alpha/CD172a antibody 31485-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols