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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SIRP Alpha/CD172a Recombinant antibody

SIRP Alpha/CD172a Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240789C6

Cat no : 83729-5-RR

Synonyms

SIRP Alpha, SIRPA, Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A

Validation Data Gallery

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  • Western Blot (WB) analysis of U-937 cells using SIRP Alpha/CD172a Recombinant antibody (83729-5-RR)

    WB analysis of U-937 using 83729-5-RR

    U-937 cells were subjected to SDS PAGE followed by western blot with 83729-5-RR (SIRP Alpha/CD172a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using SIRP Alpha/CD172a Recombinant antibody (83729-5-RR)

    IF Staining of THP-1 using 83729-5-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using SIRP Alpha/CD172a antibody (83729-5-RR, Clone: 240789C6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83729-5-RR

    Affinity and Kinetic Characterization of 83729-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83729-5-RR against Human SIRP Alpha were performed. The affinity constant is 0.497 nM.

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Tested Applications

Positive WB detected inU-937 cells
Positive IF/ICC detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83729-5-RR is SIRP Alpha/CD172a Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name signal-regulatory protein alpha
Calculated molecular weight55 kDa
Observed molecular weight 75-85 kDa
GenBank accession numberNM_080792
Gene symbol SIRP Alpha
Gene ID (NCBI) 140885
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).

Protocols

Product Specific Protocols
WB protocol for SIRP Alpha/CD172a antibody 83729-5-RRDownload protocol
IF protocol for SIRP Alpha/CD172a antibody 83729-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols