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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SIRP Alpha/CD172a Polyclonal antibody, PBS Only

SIRP Alpha/CD172a Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14482-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SIRP Alpha/CD172a Polyclonal antibody (14482-1-AP)

    WB analysis using 14482-1-AP (same clone as 14482-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14482-1-AP (SIRP Alpha/CD172a antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14482-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SIRP Alpha/CD172a Polyclonal antibody (14482-1-AP)

    WB analysis using 14482-1-AP (same clone as 14482-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14482-1-AP (SIRP Alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14482-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-12 cells using SIRP Alpha/CD172a Polyclonal antibody (14482-1-AP)

    WB analysis of PC-12 using 14482-1-AP (same clone as 14482-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 14482-1-AP (SIRP alpha antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14482-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14482-1-PBS targets SIRP Alpha/CD172a in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIRP Alpha/CD172a fusion protein Ag5865 相同性解析による交差性が予測される生物種
Full Name signal-regulatory protein alpha
Calculated molecular weight 55 kDa
Observed molecular weight 75-85 kDa
GenBank accession numberBC033092
Gene symbol SIRP Alpha
Gene ID (NCBI) 140885
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954). This antibody detects a 75-85 kDa band.