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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human SIRP Alpha/CD172a Rabbit Recombinant Antibody, PBS Only

SIRP Alpha/CD172a Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240789E1

Cat no : 98101-1-PBS

Synonyms

SIRP Alpha, SIRPA, Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human SIRP Alpha/CD172a Rabbit Recombinant An (98101-1-RR)

    FC experiment of human peripheral blood leukocytes using 98101-1-RR (same clone as 98101-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human SIRP Alpha/CD172a Rabbit Recombinant Antibody (98101-1-RR, Clone: 240789E1) or Isotype Control, and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98101-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human SIRP Alpha/CD172a Rabbit Recombinant An (98101-1-RR)

    FC experiment of human peripheral blood leukocytes using 98101-1-RR (same clone as 98101-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human SIRPA Rabbit Recombinant Antibody (98101-1-RR, Clone: 240789E1) (red) or Isotype Control (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98101-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98101-1-RR

    Affinity and Kinetic Characterization of 98101-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98101-1-RR against Human SIRP Alpha/CD172a were performed. The affinity constant is 3.39 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 98101-1-PBS is SIRP Alpha/CD172a Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name signal-regulatory protein alpha
Calculated molecular weight55 kDa
GenBank accession numberNM_080792
Gene symbol SIRP Alpha
Gene ID (NCBI) 140885
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).