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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SIRP alpha/CD172a Polyclonal antibody (31952-1-AP)

    WB analysis using 31952-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31952-1-AP (SIRP alpha/CD172a antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using SIRP alpha/CD172a Polyclonal antibody (31952-1-AP)

    IF Staining of RAW 264.7 using 31952-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using SIRP alpha/CD172a antibody (31952-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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SIRP alpha/CD172a Polyclonal antibody

SIRP alpha/CD172a Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31952-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Synonyms

mSIRP-alpha1, Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A, Brain Ig-like molecule with tyrosine-based activation motifs

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJ774A.1 cells, RAW 264.7 cells
Positive IF/ICC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31952-1-AP targets SIRP alpha/CD172a in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name signal-regulatory protein alpha
Calculated molecular weight56 kDa
Observed molecular weight110 kDa
GenBank accession numberNM_007547.4
Gene Symbol Sirpa
Gene ID (NCBI) 19261
RRIDAB_3670158
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP97797-2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).

Protocols

Product Specific Protocols
WB protocol for SIRP alpha/CD172a antibody 31952-1-APDownload protocol
IF protocol for SIRP alpha/CD172a antibody 31952-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols