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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SIRP Alpha/CD172a Recombinant antibody, PBS Only (Detector)

SIRP Alpha/CD172a Recombinant Antibody for IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240789E7

Cat no : 83729-3-PBS

Synonyms

SIRP Alpha, SIRPA, Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using SIRP Alpha/CD172a Recombinant antibody (83729-3-RR)

    IF Staining of THP-1 using 83729-3-RR (same clone as 83729-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using SIRP Alpha/CD172a antibody (83729-3-RR, Clone: 240789E7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83729-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00713-2

    Cytometric bead array standard curve of MP00713-2

    Cytometric bead array standard curve of MP00713-2, SIRP Alpha Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83729-2-PBS. Detection antibody: 83729-3-PBS. Standard: Eg0625. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 83729-3-RR

    Affinity and Kinetic Characterization of 83729-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83729-3-RR against Human SIRP Alpha were performed. The affinity constant is 1.37 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83729-3-PBS targets SIRP Alpha/CD172a as part of a matched antibody pair:

MP00713-2: 83729-2-PBS capture and 83729-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name signal-regulatory protein alpha
Calculated molecular weight55 kDa
GenBank accession numberNM_080792
Gene symbol SIRP Alpha
Gene ID (NCBI) 140885
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).