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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SIRPD Polyclonal antibody

SIRPD Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26594-1-AP

Synonyms

PTPNS1L2, SIRP delta, SIRPD

Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using SIRPD Polyclonal antibody (26594-1-AP)

    WB analysis of fetal human brain using 26594-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 26594-1-AP (SIRPD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using SIRPD Polyclonal antibody (26594-1-AP)

    IHC staining of human brain using 26594-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26594-1-AP (SIRPD antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using SIRPD Polyclonal antibody (26594-1-AP)

    IHC staining of human brain using 26594-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26594-1-AP (SIRPD antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected infetal human brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26594-1-AP is SIRPD Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIRPD fusion protein Ag24246 相同性解析による交差性が予測される生物種
Full Name signal-regulatory protein delta
Calculated molecular weight 197 aa, 22 kDa
Observed molecular weight 19 kDa
GenBank accession numberBC033502
Gene symbol SIRPD
Gene ID (NCBI) 128646
RRIDAB_2880567
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SIRPD antibody 26594-1-APDownload protocol
IHC protocol for SIRPD antibody 26594-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols