- Featured Product
- KD/KO Validated
SIRT1 Monoclonal antibody, PBS Only
SIRT1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human
Applications
WB, IHC, IF/ICC, IP, Indirect ELISA
Conjugate
Unconjugated
CloneNo.
7F6G6
Cat no : 60303-1-PBS
Synonyms
Validation Data Gallery
Tested Applications
Recommended dilution
Application | Dilution |
---|---|
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. |
Product Information
60303-1-PBS targets SIRT1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | SIRT1 fusion protein Ag17677 相同性解析による交差性が予測される生物種 |
Full Name | sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) |
Calculated molecular weight | 747 aa, 82 kDa |
Observed molecular weight | 110-130 kDa |
GenBank accession number | BC012499 |
Gene symbol | SIRT1 |
Gene ID (NCBI) | 23411 |
RRID | AB_2881417 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS Only |
Storage Conditions | Store at -80°C. |
Background Information
SIRT1, also named as SIR2L1, contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. The post-translation modified SIRT1 is a 110-130 kDa protein, which contains one deacetylase sirtuin-type domain. The 75-80 kDa SIRT1 fragment was detected to lack the carboxy-terminus (PMID:21305533). SIRT1 exists a 57-61 kDa isoform. SIRT1 may be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane. It can shuttles between nucleus and cytoplasm. SIRT1 regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signaling events relevant to cardioprotection, including: activation of endothelial nitric oxide synthase, INS receptor signaling, and autophagy. In addition SIRT1 activation elicits resistance to oxidative stress via regulation of transcription factors and co-activators such as FOXO, Hif-2a, and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.