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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SIRT1 Recombinant antibody (82790-4-RR)

    WB analysis using 82790-4-RR (same clone as 82790-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82790-4-RR (SIRT1 antibody) at dilution of 1:4700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82790-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using SIRT1 Recombinant antibody (82790-4-RR)

    WB analysis of HeLa using 82790-4-RR (same clone as 82790-4-PBS)

    WB result of SIRT1 antibody (82790-4-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SIRT1 transfected HeLa cells. This data was developed using the same antibody clone with 82790-4-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using SIRT1 Recombinant antibody (82790-4-RR)

    IP experiment of HEK-293 using 82790-4-RR (same clone as 82790-4-PBS)

    IP result of anti-SIRT1 (IP:82790-4-RR, 4ug; Detection:82790-4-RR 1:1000) with HEK-293 cells lysate 1360 ug. This data was developed using the same antibody clone with 82790-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SIRT1 Recombinant antibody (82790-4-RR)

    IF Staining of HepG2 using 82790-4-RR (same clone as 82790-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SIRT1 antibody (82790-4-RR, Clone: 2F11 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82790-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SIRT1 Recombinant antibody (82790-4-RR)

    IF Staining of HepG2 using 82790-4-RR (same clone as 82790-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SIRT1 antibody (82790-4-RR, Clone: 2F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82790-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82790-4-RR

    Affinity and Kinetic Characterization of 82790-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82790-4-RR against Human SIRT1 were performed. The affinity constant is 4.90 nM.

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  • Featured Product
  • KD/KO Validated

SIRT1 Recombinant antibody, PBS Only

SIRT1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 82790-4-PBS
Clone No.2F11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

NAD-dependent protein deacetylase sirtuin-1, hSIRT1, hSIR2, EC:2.3.1.286, EC:2.3.1.-

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82790-4-PBS targets SIRT1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SIRT1 fusion protein Ag3808 相同性解析による交差性が予測される生物種
Full Name sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
Calculated molecular weight 747 aa, 82 kDa
Observed molecular weight110-130 kDa
GenBank accession numberBC012499
Gene Symbol SIRT1
Gene ID (NCBI) 23411
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96EB6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SIRT1, also named as SIR2L1, contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. The post-translation modified SIRT1 is a 110-130 kDa protein, which contains one deacetylase sirtuin-type domain. The 75-80 kDa SirT1 fragment was detected to lack the carboxy-terminus (PMID:21305533). SirT1 exists a 57-61 kDa isoform. SIRT1 may be found in nucleolus, nuclear euchromatin, heterochromatin, and inner membrane. It can shuttles between the nucleus and cytoplasm. SIRT1 regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signaling events relevant to cardioprotection, including activation of endothelial nitric oxide synthase, INS receptor signaling, and autophagy. In addition, SIRT1 activation elicits resistance to oxidative stress via the regulation of transcription factors and co-activators such as FOXO, Hif-2a, and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.