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  • KD/KO Validated

SIRT2 Polyclonal antibody

SIRT2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 19655-1-AP

Synonyms

SIR2, Regulatory protein SIR2 homolog 2, NAD-dependent protein defatty-acylase sirtuin-2, NAD-dependent protein deacetylase sirtuin-2, EC:2.3.1.286


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Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inrat brain tissue, human skeletal muscle tissue, mouse brain tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19655-1-AP targets SIRT2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey, zebrafish, bovine, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIRT2 fusion protein Ag7756 相同性解析による交差性が予測される生物種
Full Name sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)
Calculated molecular weight 43 kDa
Observed molecular weight 37-45 kDa
GenBank accession numberBC003547
Gene symbol SIRT2
Gene ID (NCBI) 22933
RRIDAB_2878592
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as Class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging (10545947). SirT2, a mammalian homolog of Sir2, deacetylates α-tubulin at Lys40 and histone H4 at Lys16 and has been implicated in cytoskeletal regulation and progression through mitosis (12620231,16648462). SirT2 protein is mainly cytoplasmic and is associated with microtubules and HDAC6, another tubulin deacetylase (12620231). Deacetylation of α-tubulin decreases its stability and may be required for proper regulation of cell shape, intracellular transport, cell motility, and cell division (12620231,10966460). The abundance and phosphorylation state of SirT2 increase at the G2/M transition of the cell cycle, and SirT2 relocalizes to chromatin during mitosis when histone H4 Lys16 acetylation levels decrease (16648462,12697818). Overexpression of SirT2 prolongs mitosis, while overexpression of the CDC14B phosphatase results in both decreased phosphorylation and abundance of SirT2, allowing for proper mitotic exit (12697818). Thus, the deacetylation of both histone H4 and α-tubulin by SirT2 may be critical for proper chromatin and cytoskeletal dynamics required for completion of mitosis. This antibody recognizes the 37-45 KD SIRT2 proteins. This antibody is a specific antibody that it can't detect signal with SIRT2-KO samples.

Protocols

Product Specific Protocols
WB protocol for SIRT2 antibody 19655-1-APDownload protocol
IHC protocol for SIRT2 antibody 19655-1-APDownload protocol
IP protocol for SIRT2 antibody 19655-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Metab

Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.

Authors - Yi Fu
human,mouseWB,IF

Neuron

Oligodendrocytes enhance axonal energy metabolism by deacetylation of mitochondrial proteins through transcellular delivery of SIRT2.

Authors - Kelly A Chamberlain
  • KO Validated
humanWB

J Clin Invest

SIRT2 protects peripheral neurons from cisplatin-induced injury by enhancing nucleotide excision repair.

Authors - Manchao Zhang
mouseWB

Nat Commun

Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling.

Authors - Min Liu
humanWB

Nat Commun

A translational program that suppresses metabolism to shield the genome.

Authors - Nathan C Balukoff
mouseWB

Aging Cell

SIRT2-knockdown rescues GARS-induced Charcot-Marie-Tooth neuropathy.

Authors - Yingying Zhao
  • KD Validated