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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SIRT3 Recombinant antibody

SIRT3 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2P6

Cat no : 82210-1-RR

Synonyms

SIR2L3, SIR2 like protein 3, Regulatory protein SIR2 homolog 3, NAD-dependent protein deacetylase sirtuin-3, mitochondrial, hSIRT3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SIRT3 Recombinant antibody (82210-1-RR)

    WB analysis using 82210-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82210-1-RR (SIRT3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SIRT3 Recombinant antibody (82210-1-RR)

    WB analysis using 82210-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82210-1-RR (SIRT3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SIRT3 Recombinant antibody (82210-1-RR)

    IHC staining of human prostate cancer using 82210-1-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82210-1-RR (SIRT3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SIRT3 Recombinant antibody (82210-1-RR)

    IHC staining of human prostate cancer using 82210-1-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82210-1-RR (SIRT3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82210-1-RR

    Affinity and Kinetic Characterization of 82210-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82210-1-RR against Human SIRT3 were performed. The affinity constant is 0.261 nM.

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Tested Applications

Positive WB detected inHeLa cells, rat heart tissue, HepG2 cells, HT-29 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82210-1-RR targets SIRT3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SIRT3 fusion protein Ag26280 相同性解析による交差性が予測される生物種
Full Name sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
Calculated molecular weight 44 kDa
Observed molecular weight28-30 kDa
GenBank accession numberBC001042
Gene symbol SIRT3
Gene ID (NCBI) 23410
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) and SIRT3 is the only sirtuin whose increased expression has been shown to correlate with an extended life span in humans. It is localized in the mitochondrial matrix, where it regulates the acetylation levels of metabolic enzymes, including acetyl coenzyme A synthetase 2. SIRT3 is stress-responsive and its increased expression protects myocytes from genotoxic and oxidative stress-mediated cell death. full-length hSIRT3 (44 kDa) is an inert protein and that it is activated inside the mitochondria following deletion of 142 amino acids of the N-terminal segment. This processed form of SIRT3 is approximately 28 kDa and possesses deacetylase activity. The full SIRT3 is a 44 kDa protein and is processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product.

Protocols

Product Specific Protocols
WB protocol for SIRT3 antibody 82210-1-RRDownload protocol
IHC protocol for SIRT3 antibody 82210-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols