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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SIRT4 Monoclonal antibody (66543-1-Ig)

    WB analysis of HeLa using 66543-1-Ig (same clone as 66543-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66543-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using SIRT4 Monoclonal antibody (66543-1-Ig)

    WB analysis of PC-3 using 66543-1-Ig (same clone as 66543-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66543-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCCIT cells using SIRT4 Monoclonal antibody (66543-1-Ig)

    WB analysis of NCCIT using 66543-1-Ig (same clone as 66543-1-PBS)

    NCCIT cell were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66543-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using SIRT4 Monoclonal antibody (66543-1-Ig)

    WB analysis of L02 using 66543-1-Ig (same clone as 66543-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66543-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

SIRT4 Monoclonal antibody, PBS Only

SIRT4 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 66543-1-PBS
Clone No.1D5A2

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Synonyms

SIR2 like protein 4, SIR2L4, SIRT4

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66543-1-PBS targets SIRT4 in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SIRT4 fusion protein Ag17347 相同性解析による交差性が予測される生物種
Full Name sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae)
Calculated molecular weight 314 aa, 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC109319
Gene Symbol SIRT4
Gene ID (NCBI) 23409
RRIDAB_2881905
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y6E7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SIRT4, also named as NAD-dependent protein lipoamidase sirtuin-4, mitochondria, is a 314 amino acid protein, which belongs to the sirtuin family. Class II subfamily. SIRT4 is detected in vascular smooth muscle and striated muscle. SIRT4 is detected in insulin-producing beta-cells in pancreas islets of Langerhans. SIRT4 Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. It catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications and inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner. SIRT4 expression is down-regulated in a number of cancers, while overexpression reduces cell proliferation, transformation, and tumor development.