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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SIRT5 Monoclonal antibody, PBS Only

SIRT5 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F2B2

Cat no : 67257-1-PBS

Synonyms

FLJ36950, SIR2 like protein 5, SIR2L5, SIRT5

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using SIRT5 Monoclonal antibody (67257-1-Ig)

    WB analysis of HeLa using 67257-1-Ig (same clone as 67257-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 67257-1-Ig (SIRT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67257-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using SIRT5 Monoclonal antibody (67257-1-Ig)

    WB analysis of HEK-293 using 67257-1-Ig (same clone as 67257-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67257-1-Ig (SIRT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67257-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using SIRT5 Monoclonal antibody (67257-1-Ig)

    WB analysis of L02 using 67257-1-Ig (same clone as 67257-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 67257-1-Ig (SIRT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67257-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using SIRT5 Monoclonal antibody (67257-1-Ig)

    WB analysis of LNCaP using 67257-1-Ig (same clone as 67257-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67257-1-Ig (SIRT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67257-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using SIRT5 Monoclonal antibody (67257-1-Ig)

    WB analysis of K-562 using 67257-1-Ig (same clone as 67257-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 67257-1-Ig (SIRT5 antibody) at dilution of 1:4800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67257-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SIRT5 Monoclonal antibody (67257-1-Ig)

    IHC staining of human liver cancer using 67257-1-Ig (same clone as 67257-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67257-1-Ig (SIRT5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67257-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using SIRT5 Monoclonal antibody (67257-1-Ig)

    IHC staining of human heart using 67257-1-Ig (same clone as 67257-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67257-1-Ig (SIRT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67257-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using SIRT5 Monoclonal antibody (67257-1-Ig)

    IHC staining of human heart using 67257-1-Ig (same clone as 67257-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67257-1-Ig (SIRT5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67257-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using SIRT5 Monoclonal antibody (67257-1-Ig)

    IHC staining of human liver cancer using 67257-1-Ig (same clone as 67257-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67257-1-Ig (SIRT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67257-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67257-1-PBS targets SIRT5 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SIRT5 fusion protein Ag7743 相同性解析による交差性が予測される生物種
Full Name sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae)
Calculated molecular weight 34 kDa
Observed molecular weight33 kDa
GenBank accession numberBC000126
Gene symbol SIRT5
Gene ID (NCBI) 23408
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SIRT5, a member fo sirtuin family that is a mitochondrial matrix NAD(+)-dependent deacetylase and mono-ADP-ribosyltransferases. Sirtuins play important role in various cellular processed, such as aging, gene silencing and metabolism. SIRT5 mainly locates in . It is an efficient protein lysine desuccinylase and demalonylase. During prolonged fasting, it can activate CPS1, the committed and regulated enzyme of the urea cycle, and contribute to the regulation of blood ammonia levels.