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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SIRT6 Polyclonal antibody

SIRT6 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28677-1-AP

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SIRT6 Polyclonal antibody (28677-1-AP)

    WB analysis using 28677-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28677-1-AP (SIRT6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28677-1-AP targets SIRT6 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIRT6 fusion protein Ag29163 相同性解析による交差性が予測される生物種
Full Name sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
Calculated molecular weight 39 kDa
Observed molecular weight39 kDa
GenBank accession numberBC028220
Gene symbol SIRT6
Gene ID (NCBI) 51548
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The SIRT6 gene is located at chromosome 19p13.3 and contains 8 exons. The encoded protein has a total length of 355 amino acids. SIRT6 protein is a member of the Sirtuins, which is a class of NAD+-dependent protein deacetylases involved in stress resistance and metabolic homeostasis. SIRT6 also plays roles in various tumors as both an oncogene and tumor suppressor gene. A previous study in osteosarcoma reported that SIRT6 regulates the migration and invasion of tumors through the ERK1/2/MMP9 pathway. (PMID: 30675128)

Protocols

Product Specific Protocols
WB protocol for SIRT6 antibody 28677-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols