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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SIX1 Monoclonal antibody, PBS Only

SIX1 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rabbit, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2D7D1

Cat no : 67741-1-PBS

Synonyms

BOS3, DFNA23, Homeobox protein SIX1, Sine oculis homeobox homolog 1, SIX homeobox 1, SIX1, TIP39

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SIX1 Monoclonal antibody (67741-1-Ig)

    WB analysis using 67741-1-Ig (same clone as 67741-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67741-1-Ig (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67741-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SIX1 Monoclonal antibody (67741-1-Ig)

    WB analysis using 67741-1-Ig (same clone as 67741-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67741-1-Ig (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67741-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using SIX1 Monoclonal antibody (67741-1-Ig)

    WB analysis using 67741-1-Ig (same clone as 67741-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67741-1-Ig (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67741-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using SIX1 Monoclonal antibody (67741-1-Ig)

    IF Staining of A431 using 67741-1-Ig (same clone as 67741-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SIX1 antibody (67741-1-Ig, Clone: 2D7D1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67741-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67741-1-PBS targets SIX1 in WB, IF, ELISA applications and shows reactivity with Human, mouse, pig, rabbit, rat samples.

Tested Reactivity Human, mouse, pig, rabbit, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SIX1 fusion protein Ag28570 相同性解析による交差性が予測される生物種
Full Name SIX homeobox 1
Calculated molecular weight 32 kDa
Observed molecular weight30-35 kDa
GenBank accession numberBC008874
Gene symbol SIX1
Gene ID (NCBI) 6495
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

SIX1 belongs to SIX family that contains a divergent DNA-binding homeodomain and an upstream SIX domain, which may participate both in determining DNA-binding specificity and in mediating protein-protein interactions. It may be involved in limb tendon and ligament development, and specifically expressed in skeletal muscle. SIX1 plays a essential role in the development of numerous organs and shows littlt to no expression in most non-neoplastic adult tissue, yet is overexpression in a number of meoplasms where it increases cell proliferation and survival. The calcualted molecular weight of SIX1 is 32kDa, but modified SIX1 is about 35 kDa.