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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SIX6 Monoclonal antibody

SIX6 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C12E2

Cat no : 67622-1-Ig

Synonyms

Homeobox protein SIX6, Homeodomain protein OPTX2, MCOPCT2, Optic homeobox 2, OPTX2, Sine oculis homeobox homolog 6, SIX homeobox 6, SIX6, Six9

Validation Data Gallery

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  • Western Blot (WB) analysis of HUVEC cells using SIX6 Monoclonal antibody (67622-1-Ig)

    WB analysis of HUVEC using 67622-1-Ig

    HUVEC cells were subjected to SDS PAGE followed by western blot with 67622-1-Ig (SIX6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NCCIT cells using SIX6 Monoclonal antibody (67622-1-Ig)

    WB analysis of NCCIT using 67622-1-Ig

    NCCIT cells were subjected to SDS PAGE followed by western blot with 67622-1-Ig (SIX6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHUVEC cells, NCCIT cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67622-1-Ig targets SIX6 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SIX6 fusion protein Ag15496 相同性解析による交差性が予測される生物種
Full Name SIX homeobox 6
Calculated molecular weight 246 aa, 28 kDa
Observed molecular weight 30-35 kDa
GenBank accession numberBC069413
Gene symbol SIX6
Gene ID (NCBI) 4990
RRIDAB_2882824
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SIX6, also named as OPTX2 or SIX9, is a 246 amino acid protein, which is expressed in the developing and adult retina. Defects in this gene are a cause of isolated microphthalmia with cataract type 2 (MCOPCT2).

Protocols

Product Specific Protocols
WB protocol for SIX6 antibody 67622-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols