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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SKAP2 Monoclonal antibody

SKAP2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2G3G9

Cat no : 66367-1-Ig

Synonyms

PRAP, Pyk2/RAFTK associated protein, RA70, SAPS, SCAP2, SKAP 55HOM, SKAP HOM, SKAP2, SKAP55 homolog, SKAP55R

Validation Data Gallery

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  • Western Blot (WB) analysis of RAW 264.7 cells using SKAP2 Monoclonal antibody (66367-1-Ig)

    WB analysis of RAW 264.7 using 66367-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66367-1-Ig (SKAP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human spleen tissue using SKAP2 Monoclonal antibody (66367-1-Ig)

    WB analysis of human spleen using 66367-1-Ig

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66367-1-Ig (SKAP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using SKAP2 Monoclonal antibody (66367-1-Ig)

    WB analysis of pig liver using 66367-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66367-1-Ig (SKAP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SKAP2 Monoclonal antibody (66367-1-Ig)

    IHC staining of human colon cancer using 66367-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66367-1-Ig (SKAP2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SKAP2 Monoclonal antibody (66367-1-Ig)

    IHC staining of human colon cancer using 66367-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66367-1-Ig (SKAP2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using SKAP2 Monoclonal antibody (66367-1-Ig)

    IF Staining of NIH/3T3 using 66367-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using SKAP2 antibody (66367-1-Ig, Clone: 2G3G9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Tested Applications

Positive WB detected inRAW 264.7 cells, human spleen tissue, pig liver tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66367-1-Ig targets SKAP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig, rat, mouse samples.

Tested Reactivity human, pig, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SKAP2 fusion protein Ag3615 相同性解析による交差性が予測される生物種
Full Name src kinase associated phosphoprotein 2
Calculated molecular weight 41 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC036044
Gene symbol SKAP2
Gene ID (NCBI) 8935
RRIDAB_2881747
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SKAP2 (src kinase associated phosphoprotein 2), also named PRAP, contains PH domain and SH3 domain. It is localized in cytoplasm and was expressed in ubiquitously in various tissues including lung, skeletal muscle, and spleen, but not detectable in thymus and T cell lymphoma cells. SKAP2 may negatively regulate alpha-synuclein phosphorylation following cell stress. SKAP2 may be involved in B-cell and macrophage adhesion processes by coupling the B-cell receptor (BCR) to integrin activation.

Protocols

Product Specific Protocols
WB protocol for SKAP2 antibody 66367-1-IgDownload protocol
IHC protocol for SKAP2 antibody 66367-1-IgDownload protocol
IF protocol for SKAP2 antibody 66367-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols