Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of HEK-293 using 11462-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11462-1-AP (SKIV2L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 11462-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11462-1-AP (SKIV2L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11462-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 11462-1-AP (SKIV2L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 11462-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11462-1-AP (SKIV2L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 11462-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 11462-1-AP (SKIV2L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 11462-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 11462-1-AP (SKIV2L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 11462-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 11462-1-AP (SKIV2L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11462-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11462-1-AP (SKIV2L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 11462-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 11462-1-AP (SKIV2L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 11462-1-AP
IP result of anti-SKIV2L (IP:11462-1-AP, 4ug; Detection:11462-1-AP 1:600) with HepG2 cells lysate 3200ug.
IP experiment of HepG2 using 11462-1-AP
IP result of anti-SKIV2L (IP:11462-1-AP, 4ug; Detection:11462-1-AP 1:600) with HepG2 cells lysate 3600ug.
IHC staining of human stomach using 11462-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11462-1-AP (SKIV2L antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach using 11462-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11462-1-AP (SKIV2L antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

SKIV2L Polyclonal antibody

SKIV2L Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 11462-1-AP

Publications(14)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, IP, ChIP, ELISA

Synonyms

SKI2, HLP, Helicase SKI2W, EC:3.6.4.13, DDX13

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (14)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, COLO 320 cells, human kidney tissue, HepG2 cells, Jurkat cells
Positive IP detected in	HepG2 cells
Positive IHC detected in	human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 13 publications below
IHC	See 1 publications below
IF	See 2 publications below
ChIP	See 1 publications below

Product Information

11462-1-AP targets SKIV2L in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SKIV2L fusion protein Ag1977 相同性解析による交差性が予測される生物種
Full Name	superkiller viralicidic activity 2-like (S. cerevisiae)
Calculated molecular weight	1246 aa, 138 kDa
Observed molecular weight	138 kDa
GenBank accession number	BC015758
Gene Symbol	SKIV2L
Gene ID (NCBI)	6499
RRID	AB_2187472
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q15477
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The Ski complex is a multiprotein complex required for exosome-mediated RNA surveillance, including the regulation of normal mRNA and the decay of nonfunctional mRNA. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C) [PMID:22444670]. SKIV2L , one component of the Ski complex, acts as an RNA helicase with a role in exosome recruitment or activation. It is involved in the degradation of RNAs by the exosome and may have a role in autophagy [PMID:11719186].

Protocols

Product Specific Protocols
WB protocol for SKIV2L antibody 11462-1-AP	Download protocol
IHC protocol for SKIV2L antibody 11462-1-AP	Download protocol
IP protocol for SKIV2L antibody 11462-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	Nat Immunol The SKIV2L RNA exosome limits activation of the RIG-I-like receptors. Authors - Sterling C Eckard View Article
	WB	Sci Immunol The mammalian SKIV2L RNA exosome is essential for early B cell development. Authors - Kun Yang View Article
human	WB	J Clin Invest Cytoplasmic RNA quality control failure engages mTORC1-mediated autoinflammatory disease. Authors - Kun Yang View Article
human	WB	Nucleic Acids Res Dom34 mediates targeting of exogenous RNA in the antiviral OAS/RNase L pathway. Authors - Takuto Nogimori KD Validated View Article
human	WB	Nucleic Acids Res A short splicing isoform of HBS1L links the cytoplasmic exosome and SKI complexes in humans. Authors - Katarzyna Kalisiak View Article
human	WB	Proc Natl Acad Sci U S A The SKI complex is a broad-spectrum, host-directed antiviral drug target for coronaviruses, influenza, and filoviruses. Authors - Stuart Weston KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HEK-293 using 11462-1-AP

WB analysis using 11462-1-AP

WB analysis of HepG2 using 11462-1-AP

WB analysis of Jurkat using 11462-1-AP

WB analysis of COLO 320 using 11462-1-AP

WB analysis of COLO 320 using 11462-1-AP

WB analysis of COLO 320 using 11462-1-AP

WB analysis of HEK-293 using 11462-1-AP

WB analysis of human kidney using 11462-1-AP

IP experiment of HepG2 using 11462-1-AP

IP experiment of HepG2 using 11462-1-AP

IHC staining of human stomach using 11462-1-AP

IHC staining of human stomach using 11462-1-AP

SKIV2L Polyclonal antibody

SKIV2L Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The SKIV2L RNA exosome limits activation of the RIG-I-like receptors.

The mammalian SKIV2L RNA exosome is essential for early B cell development.

Cytoplasmic RNA quality control failure engages mTORC1-mediated autoinflammatory disease.

Dom34 mediates targeting of exogenous RNA in the antiviral OAS/RNase L pathway.

A short splicing isoform of HBS1L links the cytoplasmic exosome and SKI complexes in humans.

The SKI complex is a broad-spectrum, host-directed antiviral drug target for coronaviruses, influenza, and filoviruses.