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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SKIV2L Monoclonal antibody (68547-1-Ig)

    WB analysis using 68547-1-Ig (same clone as 68547-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68547-1-Ig (SKIV2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68547-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using SKIV2L Monoclonal antibody (68547-1-Ig)

    IHC staining of human placenta using 68547-1-Ig (same clone as 68547-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68547-1-Ig (SKIV2L antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68547-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using SKIV2L Monoclonal antibody (68547-1-Ig)

    IHC staining of human placenta using 68547-1-Ig (same clone as 68547-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68547-1-Ig (SKIV2L antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68547-1-PBS in a different storage buffer formulation.

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SKIV2L Monoclonal antibody, PBS Only

SKIV2L Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 68547-1-PBS
Clone No.1A9G2

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

HLP, Helicase SKI2W, EC:3.6.4.13, DDX13, 1A9G2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68547-1-PBS targets SKIV2L in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SKIV2L fusion protein Ag33679 相同性解析による交差性が予測される生物種
Full Name superkiller viralicidic activity 2-like (S. cerevisiae)
Calculated molecular weight 1246 aa, 138 kDa
Observed molecular weight138-145 kDa
GenBank accession numberBC015758
Gene Symbol SKIV2L
Gene ID (NCBI) 6499
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15477
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

The Ski complex is a multiprotein complex required for exosome-mediated RNA surveillance, including the regulation of normal mRNA and the decay of nonfunctional mRNA. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C) [PMID:22444670]. SKIV2L , one component of the Ski complex, acts as an RNA helicase with a role in exosome recruitment or activation. It is involved in the degradation of RNAs by the exosome and may have a role in autophagy [PMID:11719186].