SKP1 Polyclonal antibody

SKP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10990-2-AP

Synonyms

OCP2, OCP II, OCP 2, NET19, EMC19


☆対象製品が30%オフ!組換えタンパク質&免染用試薬 >>> キャンペーン実施中!


Tested Applications

Positive WB detected inHeLa cells, human brain tissue, MCF-7 cells, mouse brain tissue, mouse testis tissue
Positive IHC detected inhuman gliomas tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10990-2-AP targets SKP1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SKP1 fusion protein Ag1457 相同性解析による交差性が予測される生物種
Full Name S-phase kinase-associated protein 1
Calculated molecular weight 19 kDa
Observed molecular weight 19 kDa
GenBank accession numberBC009839
Gene symbol SKP1
Gene ID (NCBI) 6500
RRIDAB_2187492
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SKP1(S-phase kinase-associated protein 1) is also named as EMC19, OCP2, SKP1A, TCEB1L,belongs to the SKP1 family.It is an essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.

Protocols

Product Specific Protocols
WB protocol for SKP1 antibody 10990-2-APDownload protocol
IHC protocol for SKP1 antibody 10990-2-APDownload protocol
IF protocol for SKP1 antibody 10990-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Elife

Elevated FBXO45 promotes liver tumorigenesis through enhancing IGF2BP1 ubiquitination and subsequent PLK1 upregulation.

Authors - Xiao-Tong Lin
humanWB,IHC

Pharmacol Res

Brusatol has therapeutic efficacy in non-small cell lung cancer by targeting Skp1 to inhibit cancer growth and metastasis.

Authors - Shangping Xing
humanWB

Mol Nutr Food Res

Ethanol Extract of Citrus grandis 'Tomentosa' Exerts Anticancer Effects by Targeting Skp2/p27 Pathway in Non-Small Cell Lung Cancer

Authors - Da Huang
humanWB

J Cell Physiol

The Ubiquitin Ligase SCF(FBXW7α) Promotes ATA3 Degradation.

Authors - Nan Song
humanIHC, WB

J Exp Clin Cancer Res

Long non-coding RNA CCDC183-AS1 acts AS a miR-589-5p sponge to promote the progression of hepatocellular carcinoma through regulating SKP1 expression.

Authors - He Zhu
humanWB

bioRxiv

Recruitment of FBXO22 for Targeted Degradation of NSD2

Authors - David Y Nie

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

WEI (Verified Customer) (05-11-2022)

Weak band but at correct size.

  • Applications: Western Blot
  • Cell Tissue Type: Mouse Heart