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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SKP1 Monoclonal antibody, PBS Only

SKP1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D5G4

Cat no : 67745-1-PBS

Synonyms

EMC19, OCP 2, OCP II, OCP2, Organ of Corti protein 2, Organ of Corti protein II, p19A, p19skp1, SIII, SKP1, SKP1A, TCEB1L

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SKP1 Monoclonal antibody (67745-1-Ig)

    WB analysis using 67745-1-Ig (same clone as 67745-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67745-1-Ig (SKP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67745-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SKP1 Monoclonal antibody (67745-1-Ig)

    IF Staining of MCF-7 using 67745-1-Ig (same clone as 67745-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SKP1 antibody (67745-1-Ig, Clone: 1D5G4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67745-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67745-1-PBS targets SKP1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SKP1 fusion protein Ag30709 相同性解析による交差性が予測される生物種
Full Name S-phase kinase-associated protein 1
Calculated molecular weight 19 kDa
Observed molecular weight18-20 kDa
GenBank accession numberBC009839
Gene symbol SKP1
Gene ID (NCBI) 6500
RRIDAB_2918514
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SKP1(S-phase kinase-associated protein 1) is also named as EMC19, OCP2, SKP1A, TCEB1L,belongs to the SKP1 family.It is an essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.